Laboratory Checklist Template

Use the Laboratory Checklist Template to conduct inspections, ensuring compliance with safety measures and standard operational procedures in all areas.​

Laboratory Checklist Template



Emergency Preparedness

1. Is the eyewash/safety shower in good working order, with covers in place, and is the pathway unobstructed? Is the eyewash flushed monthly, and is the date of flushing documented? Are personnel aware of the eyewash/shower location and knowledgeable about how to operate the equipment?


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2. Is a chemical and biological spill kit available for minor spills?


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3. Are first aid medications and antidotes available, and are they within their expiration dates (e.g., calcium gluconate for HF exposure)?


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4. Is access to electrical breaker panels and emergency shutoff controls unobstructed, with a 3-foot clearance?


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5. Is the registration current, and are emergency contact numbers and location details posted?


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6. Are evacuation routes posted in common hallways, and do personnel know the evacuation procedure, meeting site, and location of the nearest fire alarm?


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Fire Protection

1. Are sprinkler heads unobstructed, with an 18-inch clearance below them throughout the entire room (or a 24-inch clearance for non-sprinklered areas)?


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2. Are fire extinguishers available, appropriate for the type of hazard, with inspection dates recorded, and is there unobstructed access with a 3-foot clearance?


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3. Are all fire-rated doors self-closing and kept closed?


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4. Are fire alarm strobes visible from all locations?


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General Practice

1. Are caution signs present on doors and equipment indicating specific hazards (e.g., UV, Laser, Radiation, Biohazard, noise, high voltage)?


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2. Are food and household products used in chemical or hazard areas labeled as "not for human consumption" or "lab use only"?


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3. Are household appliances (refrigerators, microwaves, blenders, grinders, etc.) labeled for "lab use only"?


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4. Is there no evidence of eating, drinking, smoking, applying cosmetics, or mouth pipetting inside the space?


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5. Are entry doors kept closed at all times and locked when unoccupied to maintain security?


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Safety Training

1. s safety training administered by SRM (Vivid, Blackboard) current?


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2. Is safety training provided directly by the supervisor documented (SOP, training checklist, etc.)?


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3. Is the hazard assessment training determination form submitted and current for potential hazards?


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Engineering Controls & Ventilation

1. Is the chemical fume hood (CFH) used for chemical or equipment storage, with the airfoil and rear baffle unobstructed, and excess equipment mounted to aid in air circulation?


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2. Is the CFH sash at or below the minimum height (18 inches) when in use and kept closed when not in use?


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3. Is access to CFH/LEV unobstructed?


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4. Is the fume hood inspection certification currently performed annually by SRM?


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5. Is local exhaust ventilation (LEV) in use and in good working order (e.g., snorkel, dust collector)?


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6. Is the Biological Safety Cabinet (BSC) certified, cleaned/decontaminated, not used with volatile chemicals or open flame, and with vacuum lines equipped with filters and disinfectant traps, including HEPA filter replacement?


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Gas Cylinders

1. Are cylinders properly chained, secured, and clearly labeled with contents, and is the storage in a dry, ventilated, fire-resistant location with caution signs posted for the gas type hazard (flammable, toxic, oxidizer, etc.)?


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2. Do reserve cylinders have cylinder caps, and are empty cylinders labeled and stored separately?


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3. Are incompatible cylinders stored separately (e.g., oxygen/flammable) at a proper distance from firewalls (20’ or 5’)?


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4. Is cylinder equipment (regulator, tubing, etc.) compatible with the gas and in proper working order?


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Chemical Management

1. Is the Chemical Hygiene Plan (CHP) available to personnel working with hazardous chemicals, and is the lab-specific CHP current and documented?


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2. Are Standard Operating Procedures (SOPs) documented for particularly hazardous chemicals and high-risk procedures?


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3. Is the chemical inventory submitted annually?


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4. Are Safety Data Sheets (SDS) available in print or electronic format, and do personnel know how to access them when needed?


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5. Are chemical containers labeled with contents, appropriate hazard warnings, and expiration dates?


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6. Are time-sensitive chemicals (e.g., peroxide formers) labeled with the date opened and removed as hazardous waste when they expire?


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7. Is chemical storage well-organized, and are incompatible materials segregated (oxidizers/flammables, acids/bases)?


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8. Are hazardous materials/liquids stored below eye level (not on the floor) and in secondary containers?


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9. Are chemicals stored in compatible containers and cabinets (e.g., acids in non-metal cabinets, flammables in approved cabinets, refrigeration)?


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10. Are chemical containers kept closed?


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11. Are unnecessary, unused, or outdated materials removed for hazardous waste disposal?


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12. Are flammable liquid storage cabinets labeled, kept closed, and with no materials stored on top of them?


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Waste Management

1. Are hazardous waste containers labeled with contents, accumulation start date, and generator information?


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2. Are waste containers kept closed to prevent off-gassing discharge?


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3. Are hazardous wastes removed for disposal before the 12-month accumulation limit?


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4. Are secondary containers used to store glass waste collection bottles to capture leaks/spills?


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5. Are syringes and other sharps waste disposed of in approved sharps containers, labeled as "hazardous" or "non-contaminated" sharps?


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6. Are needles not re-capped, bent, or broken, and are they disposed of immediately in the appropriate container?


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7. Is biological waste collected in approved bags and disposed of on time?


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Equipment Safety

1. Do rotating machinery and high-temperature devices have approved safeguards, with working safety switches and emergency stops?


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2. Is safety training for hazardous equipment documented (e.g., SOP, training checklist, badge system)?


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3. Are ladders labeled with approved warning stickers and inspected before each use?


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4. Is radiation-producing equipment registered with SRM and the NC radiation protection section?


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Electrical Safety

1. Is the equipment free of frayed or damaged wiring?


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2. Are extension cords not being used as permanent wiring?


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3. Are power strips suitable for the load involved and plugged directly into the outlet (not "daisy-chained")?


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Biological Safety

1. Is approval documented for work requiring additional registration (e.g., Animal research with IACUC, rDNA with IBC)?


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2. Is the inventory of biological agents submitted annually to SRM?


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3. Is an approved disinfectant in use, and are bleach solutions freshly prepared within 24 hours?


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Cold Room Safety

1. Does the cold room have an emergency release mechanism?


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2. Is cardboard not stored in the cold room?


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3. Unless adequate ventilation is present, are asphyxiates and hazardous gases used in cold rooms?


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4. Is general housekeeping observed, and is the area organized, uncluttered, and sanitary?


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5. Are expired/abandoned samples not stored in the cold room?


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Laser Safety

1. Is the Laser Standard Operating Procedure (SOP) available to authorized users, and is the training documented?


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2. Is the laser (Class 3b or 4) registered with SRM?


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3. Is access restricted to prevent accidental exposure while the laser is operating, and is the protective housing intact to prevent exposure?


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4. Are laser-controlled areas posted with appropriate caution signs?


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5. Are beam stops present at the end of all beam paths, and are they non-combustible?


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6. Are approved safety glasses available?


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Housekeeping

1. Are surfaces clean and decontaminated after work is performed (no chemical residue, dust, biohazards, etc.)?


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2. Are general work spaces, storage areas, and benchtops uncluttered and orderly?


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3. Are aisles and exits free from obstruction, such as slip, trip, or fall hazards?


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4. Are there glass containers stored on the floor?


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5. Are trash bags & sharps containers removed when full?


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Personal Protective Equipment (PPE)

1. Is hand protection available, matched to hazards, and in use?


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2. Is eye protection available and used as required?


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3. Are lab coats, Tyvek garments, etc., available and used when necessary?


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4. Is the use of respirators registered with SRM and properly maintained?


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5. Are personnel wearing appropriate attire ( no shorts, tank tops, or open-toed shoes)?


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6. Is head, foot, hearing, and fall protection available and used if required?


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7. Is PPE Hazard Assessment documented?


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8. Is shared PPE sanitized before and after use?


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Checklist by GoAudits.com – Please note that this checklist is intended as an example. We do not guarantee compliance with the laws applicable to your territory or industry. You should seek professional advice to determine how this checklist should be adapted to your workplace or jurisdiction.

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