Ambulance Deep Clean Checklist

Use the Ambulance Deep Clean Checklist to disinfect all surfaces, equipment, and compartments, ensuring a safe, sanitary environment for patients and crew.

Ambulance Deep Clean Checklist



Cabin & Driver’s Area

1. Have all dashboard surfaces, controls, and panels been wiped with disinfectant?


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2. Are seats, seat belts, and floor mats cleaned and disinfected thoroughly?


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3. Are touchpoints such as steering wheel, gear shift, and door handles sanitized?


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4. Have the windows and mirrors been cleaned inside and out?


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5. Are air vents and climate control filters inspected and cleaned or replaced?


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Patient Compartment – Surfaces & Fixtures

1. Are stretcher rails, mattress surfaces, and restraints cleaned with hospital-grade disinfectant?


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2. Have sidewalls, ceilings, counters, and shelving units been wiped and sanitized?


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3. Are all door handles, grab bars and storage handles disinfected?


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4. Has the suction unit, if present, been cleaned and disinfected according to protocol?


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5. Are drawers, compartments, and equipment bins emptied and cleaned inside and out?


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Medical Equipment & Supplies

1. Have reusable equipment like BP cuffs, stethoscopes, and thermometers been disinfected?


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2. Are defibrillators, monitors, and oxygen regulators wiped down and checked for cleanliness?


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3. Have sharps containers been inspected, emptied if full, and replaced safely?


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4. Are used or expired medical supplies disposed of properly and restocked?


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Infection Control & Biohazard Disposal

1. Have all biohazard bags and waste been removed and disposed of following infection control guidelines?


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2. Are linens and uniforms replaced and sent for proper laundering?


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3. Has the ambulance been ventilated to eliminate lingering odors or airborne contaminants?


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4. Are mops, buckets, and cleaning tools disinfected or changed after use?


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Flooring, Ceilings & Hidden Surfaces

1. Has the entire floor been scrubbed, with corners and edges disinfected?


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2. Are ceiling panels wiped clean, especially near air vents or light fittings?


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3. Have hard-to-reach areas under seats or around equipment been checked and cleaned?


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Final Checks & Restocking

1. Is the ambulance restocked with all required clean supplies and PPE?


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2. Have all used cleaning products been safely disposed of?


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3. Was the deep clean documented with the date, time, and initials of the person responsible?


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4. Has a supervisor or senior medic verified cleanliness and readiness for service?


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Checklist by GoAudits.com – Please note that this checklist is intended as an example. We do not guarantee compliance with the laws applicable to your territory or industry. You should seek professional advice to determine how this checklist should be adapted to your workplace or jurisdiction.

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